Journal: Journal of Oncology

Article Title: CircMAT2B Induced by TEAD1 Aggravates the Warburg Effect and Tumorigenesis of Oral Squamous Cell Carcinoma through the miR-942-5p/HSPD1 Axis

doi: 10.1155/2022/7574458

Figure Lengend Snippet: Characterization of circMAT2B in OSCC. (a) The exonic information of circMAT2B. (b) Relative expression of circMAT2B in HOK and OSCC cell lines was measured by qRT-PCR. (c) and (d) Cal-27 (d) and HSC-6 (d) cells were treated with actinomycin D and relative RNA levels were detected by qRT-PCR at the indicated time. (e) and (f) Total RNAs from Cal-27 (e) and HSC-6 (f) cells were treated with RNase R or mock and relative RNA levels were detected by qRT-PCR. G-H: Cellular RNA fractionation was conducted to assess circMAT2B distribution in Cal-27 (g) or HSC-6 (h). GAPDH or U6 was used as an internal control. Each experiment was performed three times. Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: OSCC cell lines (HOK, SCC-9, SCC-15, Cal-27, HSC-3, and HSC-6) were commercially procured from the American Tissue Culture Collection (Manassas, VA, USA) and cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS) (Gibco, USA) in a 37°C-humidity environment with 5% CO 2 .

Techniques: Expressing, Quantitative RT-PCR, Fractionation, Control

Journal: Journal of Oncology

Article Title: CircMAT2B Induced by TEAD1 Aggravates the Warburg Effect and Tumorigenesis of Oral Squamous Cell Carcinoma through the miR-942-5p/HSPD1 Axis

doi: 10.1155/2022/7574458

Figure Lengend Snippet: CircMAT2B knockdown inhibits OSCC tumorigenesis and the Warburg effect. (a) and (b) OSCC cells Cal-27 and HSC-6 were infected with Sh-NC, Sh-circMAT2B#1, and Sh-circMAT2B#2, respectively. CircMAT2B (a) and MAT2B (b) expression was measured by qRT-PCR. (c) and (d) CircMAT2B knockdown or normal control Cal-27 or HSC-6 cells were subjected to EdU assay for proliferation ability detection (c) and analysis (d). (e) and (f) transwell migration assay was performed to evaluate migration level of circMAT2B knockdown or normal control Cal-27 or HSC-6 cells (e) and (f). (g) and (h) transwell invasion assay was performed to evaluate the invasion of circMAT2B knockdown or normal control Cal-27 or HSC-6 cells (g) and (h). (i)–(k) warburg effect relative glucose uptake (i), lactate production (j), and ATP levels (k) were detected. Each experiment was performed three times. Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: OSCC cell lines (HOK, SCC-9, SCC-15, Cal-27, HSC-3, and HSC-6) were commercially procured from the American Tissue Culture Collection (Manassas, VA, USA) and cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS) (Gibco, USA) in a 37°C-humidity environment with 5% CO 2 .

Techniques: Knockdown, Infection, Expressing, Quantitative RT-PCR, Control, EdU Assay, Transwell Migration Assay, Migration, Transwell Invasion Assay

Journal: Journal of Oncology

Article Title: CircMAT2B Induced by TEAD1 Aggravates the Warburg Effect and Tumorigenesis of Oral Squamous Cell Carcinoma through the miR-942-5p/HSPD1 Axis

doi: 10.1155/2022/7574458

Figure Lengend Snippet: MiR-942-5p overexpression suppresses tumorigenesis and the Warburg effect in OSCC. (a): MiR-942-5p overexpression cell models were generated by infecting miR-942-5p mimic or its normal control into Cal-27 or HSC-6 cells and the expression of miR-942-5p was measured by qRT-PCR. (b) and (c): cell proliferation was detected by EdU assay (b) and (c). (d) and (e): cell migration was assessed by transwell migration assay (d) and (e). (f) and (g): cell invasion was measured by the transwell invasion assay (f) and (g). (h)–(j): The warburg effect relative glucose uptake (h), lactate production (i), and ATP levels (j) in OSCC cells. Each experiment was performed three times. Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: OSCC cell lines (HOK, SCC-9, SCC-15, Cal-27, HSC-3, and HSC-6) were commercially procured from the American Tissue Culture Collection (Manassas, VA, USA) and cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS) (Gibco, USA) in a 37°C-humidity environment with 5% CO 2 .

Techniques: Over Expression, Generated, Control, Expressing, Quantitative RT-PCR, EdU Assay, Migration, Transwell Migration Assay, Transwell Invasion Assay

Journal: Journal of Oncology

Article Title: CircMAT2B Induced by TEAD1 Aggravates the Warburg Effect and Tumorigenesis of Oral Squamous Cell Carcinoma through the miR-942-5p/HSPD1 Axis

doi: 10.1155/2022/7574458

Figure Lengend Snippet: CircMAT2B accelerates OSCC progression through the miR-942-5p/HSPD1 axis. (a) and (b): cell models were constructed by transfecting Sh-NC, Sh-circMAT2B#1, Sh-circMAT2B#1 + LV-NC, Sh-circMAT2B#1 + LV-HSPD1 into Cal-27 or HSC-6 cells as indicated, transfection efficiency was assessed by qRT-PCR (a) and Western blotting (b). (c) and (d): Cell proliferation was detected by the EdU assay (c) and (d). (e) and (f) cell migration was assessed by the transwell migration assay (e) and (f). (g) and (h) cell invasion was measured by the transwell invasion assay (f) and (h). (i)–(k) Warburg effect relative glucose uptake (i), lactate production (j), and ATP levels (k) in OSCC cells. Each experiment was performed three times. Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: OSCC cell lines (HOK, SCC-9, SCC-15, Cal-27, HSC-3, and HSC-6) were commercially procured from the American Tissue Culture Collection (Manassas, VA, USA) and cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS) (Gibco, USA) in a 37°C-humidity environment with 5% CO 2 .

Techniques: Construct, Transfection, Quantitative RT-PCR, Western Blot, EdU Assay, Migration, Transwell Migration Assay, Transwell Invasion Assay

Journal: World Journal of Surgical Oncology

Article Title: LncRNA NEAT1 mediates progression of oral squamous cell carcinoma via VEGF-A and Notch signaling pathway

doi: 10.1186/s12957-020-02028-x

Figure Lengend Snippet: LncRNA NEAT1 highly expressed in OSCC cell lines and promoted proliferation and EMT but inhibited apoptosis. a Expressions of lncRNA NEAT1 in HOEC, SCC-25, TSCC1, and CAL-27 cell lines were examined with RT-qPCR with normalizing to GAPDH and the statistical test was comparing each of the OSCC cell lines to the HOEC cell line, ** P < 0.05. b After suppression, levels of siNEAT1-1, siNEAT1-2, and siNC were checked by RT-qPCR with normalizing to GAPDH and the statistical test was comparing siNEAT1-1 and siNEAT1-2 to siNC, ** P < 0.05. c CCK-8 was assessed to measure cell viabilities in TSCC1 cell line with siNC and siNEAT1-2 and the statistical test was comparing siNEAT1-2 to siNC, ** P < 0.05. d Apoptosis rate of cells in TSCC1 cell line with transfected siNEAT1-2 and siNC were validated using flow cytometry and the statistical test was comparing siNEAT1-2 to siNC, ** P < 0.05. e Expressions of E-cadherin, N-cadherin, Vimentin, and Snail were checked with RT-qPCR with normalizing to GAPDH and the statistical test was comparing E-cadherin, N-cadherin, Vimentin, and Snail in siNEAT1-2 group to E-cadherin, N-cadherin, Vimentin, and Snail in siNC group, ** P < 0.05. Each experiment was repeated three times

Article Snippet: The human oral epithelial cell line HOEC and human OSCC cell lines (SCC-25, TSCC1, and CAL-27) were all acquired from American Type Culture Collection (ATCC, USA).

Techniques: Quantitative RT-PCR, CCK-8 Assay, Transfection, Flow Cytometry

Journal: World Journal of Surgical Oncology

Article Title: LncRNA NEAT1 mediates progression of oral squamous cell carcinoma via VEGF-A and Notch signaling pathway

doi: 10.1186/s12957-020-02028-x

Figure Lengend Snippet: VEGF-A expressed higher in OSCC cell lines with accelerating proliferation and EMT. a RNA expressions of VEGF-A were measured in HOEC, SCC-25, TSCC1, and CAL-27 cell lines using RT-qPCR with normalizing to GAPDH and the statistical test was comparing each of the OSCC cell lines to the HOEC cell line, ** P < 0.05. b RT-qPCR was assessed to evaluated level of VEGF-A after inhibition normalized with GAPDH and the statistical test was comparing siVEGF-A to siNC, ** P < 0.05. c Cell viabilities were checked by CCK-8 in TSCC1 cell line with suppressed VEGF-A with the statistical test comparing siVEGF-A to siNC, ** P < 0.05. d RT-qPCR was performed to check RNA levels of E-cadherin, N-cadherin, Vimentin, and Snail with normalizing to GAPDH and the statistical test was comparing E-cadherin, N-cadherin, Vimentin, and Snail in siVEGF-A group to E-cadherin, N-cadherin, Vimentin, and Snail in siNC group, ** P < 0.05. Each experiment was repeated three times

Article Snippet: The human oral epithelial cell line HOEC and human OSCC cell lines (SCC-25, TSCC1, and CAL-27) were all acquired from American Type Culture Collection (ATCC, USA).

Techniques: Quantitative RT-PCR, Inhibition, CCK-8 Assay

Journal: World Journal of Surgical Oncology

Article Title: LncRNA NEAT1 mediates progression of oral squamous cell carcinoma via VEGF-A and Notch signaling pathway

doi: 10.1186/s12957-020-02028-x

Figure Lengend Snippet: LncRNA NEAT1 accelerated proliferation and EMT and repressed apoptosis of OSCC cells through activating Notch signaling pathway. a Expressions of Notch1, Notch2, and Jagged1 with overexpressed NEAT1-2 and VEGF-A were evaluated by RT-qPCR normalized with GAPDH and the statistical test was comparing oeNEAT1-2 and oeNEAT1-2+oeVEGF-A to oeNC, ** P < 0.05. b Cell viabilities were checked in TSCC1 cells with overexpressed NEAT-1, overexpressed VEGF-A and IMR-1 via CCK-8 with the statistical test was comparing oeNEAT1-2, oeNEAT1-2+oeVEGF-A, and oeNEAT1-2+oeVEGF-A+IMR-1 to oeNC, ** P < 0.05. c Flow cytometry was performed to measure apoptosis rate of TSCC1 cell line with overexpressed NEAT1-2 and VEGF-A and added IMR-1 with the statistical test was comparing oeNEAT1-2, oeNEAT1-2+oeVEGF-A, and oeNEAT1-2+oeVEGF-A+IMR-1 to oeNC, ** P < 0.05. d RNA levels of E-cadherin, N-cadherin, Vimentin, and Snail were detected with overexpressed NEAT1-2, overexpressed VEGF-A and IMR-1 using RT-qPCR with normalizing to GAPDH and the statistical test was comparing E-cadherin, N-cadherin, Vimentin, and Snail in oeNEAT1-2, oeNEAT1-2+oeVEGF-A, and oeNEAT1-2+oeVEGF-A+IMR-1 group to E-cadherin, N-cadherin, Vimentin, and Snail in oeNC group, ** P < 0.05. Each experiment was repeated three times

Article Snippet: The human oral epithelial cell line HOEC and human OSCC cell lines (SCC-25, TSCC1, and CAL-27) were all acquired from American Type Culture Collection (ATCC, USA).

Techniques: Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry

Journal: Cancer Nanotechnology

Article Title: Modulating tumour metabolism enhances gold nanoparticle radiosensitisation in HPV-negative head and neck cancer

doi: 10.1186/s12645-023-00185-8

Figure Lengend Snippet: Fig. 1 Cell uptake of AuNPs in FaDu (a), CAL27 (b) and CAL33 (c) cells under atmospheric (21% O2) or hypoxic (0.5% O2) conditions. One-way ANOVA followed by Turkey’s multiple tests was used to compare within different concentrations, * p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data were obtained from three independent experiments performed in triplicate ± SEM

Article Snippet: CAL27 cells were obtained from the American Type Culture Collection [ATCC), and FaDu and CAL33 cells obtained from DSMZ [Brauschweig, Germany).

Techniques:

Journal: Cancer Nanotechnology

Article Title: Modulating tumour metabolism enhances gold nanoparticle radiosensitisation in HPV-negative head and neck cancer

doi: 10.1186/s12645-023-00185-8

Figure Lengend Snippet: Fig. 2 Intracellular uptake and localisation of AuNPs within FaDu, CAL27 and CAL33 cells under atmospheric oxygen conditions using Cytoviva darkfield/hyperspectral imaging. Cells were treated with AuNPs for 24 h, fixed and mounted with DAPI before imaging. Representative images include enhanced dark field and the corresponding hyperspectral images, overlaid with a spectral angle map (SAM) representing AuNPs in red

Article Snippet: CAL27 cells were obtained from the American Type Culture Collection [ATCC), and FaDu and CAL33 cells obtained from DSMZ [Brauschweig, Germany).

Techniques: Imaging

Journal: Cancer Nanotechnology

Article Title: Modulating tumour metabolism enhances gold nanoparticle radiosensitisation in HPV-negative head and neck cancer

doi: 10.1186/s12645-023-00185-8

Figure Lengend Snippet: Fig. 4 Clonogenic radiation survival curves for FaDu (a and d), CAL27 (b and e) and CAL33 (c and f) cells treated with either AuNPs (100 μg), atovaquone (AQ, 30 μM) or the combination of both under normoxic (a, b and c) and hypoxic (d, e and f) conditions. Two-way ANOVA with Turkey multiple comparison test was used to compare within different treatments. Significant differences are represented by *p < 0.05, **p < 0.01, ***p < 0.001. Data were obtained from three independent experiments performed in triplicate ± SEM

Article Snippet: CAL27 cells were obtained from the American Type Culture Collection [ATCC), and FaDu and CAL33 cells obtained from DSMZ [Brauschweig, Germany).

Techniques: Comparison

Journal: Cancer Nanotechnology

Article Title: Modulating tumour metabolism enhances gold nanoparticle radiosensitisation in HPV-negative head and neck cancer

doi: 10.1186/s12645-023-00185-8

Figure Lengend Snippet: Fig. 6 Annexin V/PI staining for apoptosis in FaDu (a, d), CAL27 (b, c) and CAL33 (e, f) cells treated with AuNPs (100 μg), atovaquone (AQ 30 μM) or radiation (4 Gy) or the combination. Cells were maintained under atmospheric oxygen conditions (21% O2—panels a, b and c) or hypoxia (0.5% O2—panels d, e and f). Data presented are from at least three independent replicates performed in triplicate ± SEM. One-way ANOVA followed by Tukey’s multiple comparisons test was used to compare within different treatments, significant differences are represented by *p < 0.05, **p < 0.01, respectively

Article Snippet: CAL27 cells were obtained from the American Type Culture Collection [ATCC), and FaDu and CAL33 cells obtained from DSMZ [Brauschweig, Germany).

Techniques: Staining